Difference media

 Differential media : 


* Media containing reagents or chemical which differentiate growth of one kind of organism for other kinds are known as differential media.


* The common example of differential media are Mac-Conkey's agar, Eosin methylene blue agar and blood agar.


* The contain dyes, reagents or chemicals which allow the observe to distinguish between types of bacterial colonies developed after incubation.


* For examples, if raw sewage is streaked or spread on eosinmethylene blue (EMB) agar some bacteria produce brilliant green colonies, others gummy, pink colonies with dark centres.


* Blood agar medium is a good differential medium.


* If a mixture of bacteria is inoculated on a blood agar medium, some of the bacteria may hemolyse (destroy) the red blood cells while others do not show hemolytic reactions.


* The observer may see a clear zone of hemolyzed red blood cells around certain colonies colonies of bacteria while the non-hemolyzed bacterial colonies do not develop such a clear zone around them.










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